Transgenic Microalgae as Green Cell Factories

Advances in Experimental Medicine and Biology Band 616

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Beschreibung


Microalgae have been largely cultured and commercialized as food and feed

additives, and their potential as source of high-added value compounds is well known.

But, in contrast to the large number of genetically modified bacteria, yeast and even

higher plants, only a few species of microalgae have been genetically transformed

with efficiency. Initial difficulties in the expression of foreign genes in microalgae

have been progressively overcome, and powerful molecular tools for their genetic

engineering are now on hand. A considerable collection of promoters and selectable

marker genes and an increasing number of genomic or cDNA sequences have become

available in recent years. More work is needed to transform new species of

microalgae, specially those that have commercial value, so that it would be possible

to increase the productivity of traditional compounds or synthesize novel ones. Silencing

transgenes remains as an important limitation for stable expression of foreign

genes. This problem is not unique to microalgae since it has also been observed

in plants, animals and fungi. A better understanding of the mechanisms that control

the regulation of gene expression in eukaryotes is therefore needed.

In this book a group of outstanding researchers working on different areas of

microalgae biotechnology offer a global vision of the genetic manipulation of

microalgae and their applications.

Produktdetails

Einband Taschenbuch
Herausgeber Rosa León, Aurora Galván Cejudo, Emilio Fernández
Seitenzahl 131
Erscheinungsdatum 25.11.2014
Sprache Englisch
ISBN 978-1-4899-9132-4
Verlag Springer Us
Maße (L/B/H) 25,4/17,8/0,8 cm
Gewicht 294 g
Auflage 2007

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  • CONTENTS 1. NUCLEAR TRANSFORMATION OF EUKARYOTIC MICROALGAE: HISTORICAL OVERVIEW, ACHIEVEMENTS AND PROBLEMS ................................................ 1 Rosa León and Emilio Fernández Abstract ............................................................................................................................... 1 Introduction ......................................................................................................................... 1 Microalgae Groups Transformed ....................................................................................... 2 Methods for Microalgae Transformation .......................................................................... 4 Characteristics of the Transformation Process ................................................................. 6 DNA Constructions Used in Transformation .................................................................... 6 Difficulties for Stable Expression of the Transgenes ........................................................ 8 Concluding Remarks ........................................................................................................... 8 2. TRANSFORMATION OF CYANOBACTERIA......................................... 12 Agustín Vioque Abstract .............................................................................................................................. 12 Introduction ....................................................................................................................... 12 Transformation of Cyanobacteria .................................................................................... 13 Applications ....................................................................................................................... 14 3. MOLECULAR BIOLOGY AND THEBIOTECHNOLOGICAL POTENTIAL OF DIATOMS .............................................................. 23 Peter Kroth Abstract .............................................................................................................................. 23 Diatom Biology .................................................................................................................. 23 Genetic Manipulation of Diatoms .................................................................................... 25 Biochemistry of Diatoms and Technological Applications ............................................. 29 Synthesis of Fatty Acids .................................................................................................... 29 Biomineralization .............................................................................................................. 30 Concluding Remarks ......................................................................................................... 31 00LeonAEMB 9 8/13/07, 9:53 AM x Contents 4. TOOLS AND TECHNIQUES FOR CHLOROPLAST TRANSFORMATION OF CHLAMYDOMONAS........................... 34 Saul Purton Abstract .............................................................................................................................. 34 Introduction ....................................................................................................................... 34 Delivery of DNA into the Chloroplast Compartment .................................................... 36 Integration of Transforming DNA ................................................................................... 37 Polyploidy and the Problems of Heteroplasmy ............................................................... 39 Selection Strategies ....................................................